Manly captain Daly Cherry-Evans will skipper the Prime Minister's XIII to face Papua New Guinea.
The squad includes eight players who have represented the Kangaroos and 12 debutants.
"Playing in Papua New Guinea is a privilege, as there really is nothing like it anywhere in the world," PM's XIII men's coach Mal Meninga said.
"We've struck a good balance by including some of our more experienced representative players to play alongside some very talented young players," PM's XIII men's Coach Mal Meninga said.
"A strong performance in Port Moresby on Saturday week will go some way to helping decide the final squad we take across to New Zealand for the upcoming Test Matches."
Australian PM's XIII Men's Squad
1. Corey Allan (Broncos)
2. Zac Lomax (Dragons)
3. Jesse Ramien (Sharks)
4. Brian Kelly (Sea Eagles)
5. Nick Cotric (Raiders)
6. Jake Clifford (Cowboys)
7. Daly Cherry-Evans (c) (Sea Eagles)
8. David Klemmer (Bulldogs)
9. Ben Hunt (Dragons)
10. Jordan McLean (Cowboys)
11. Tyson Frizell (Dragons)
12. Tyrone Peachey (Panthers)
13. Jake Trbojevic (Sea Eagles)
14. Aaron Woods (Sharks)
15. Reagan Campbell-Gillard (Panthers)
16. Matt Prior (Sharks)
17. Chad Townsend (Sharks)
18. Gehamat Shibasaki (Broncos)
19. Reece Robson (Dragons)
20. Enari Tuala (Cowboys)