The classy centre’s injury occurred during a captain’s run last week.

Lyon was due to play his final home match at Brookvale Oval on Saturday afternoon against Canberra but will  miss the fixture which was expected to draw close to a capacity crowd with fans set to farewell the Manly legend.

The two-time premiership winner considers himself unlikely to make it back for the final round as well.

"I've been trying to get back for the last game, but it's not looking the best. A bit of a hamstring injury which is hard to shake. A little bit frustrating, but it is what it is," he said on Tuesday.